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Image Search Results
Journal: International Journal of Nanomedicine
Article Title: Engineered hepatitis B virus surface antigen L protein particles for in vivo active targeting of splenic dendritic cells
doi: 10.2147/IJN.S32813
Figure Lengend Snippet: Flow cytometric analysis of ex vivo attachment of α-DC-ZZ-BNCs complexes to splenic DCs. ( A ) Accumulation of Cy5-labeled α-DC-ZZ-BNC complexes to isolated splenic DCs. Splenic DCs were incubated with each Cy5-labeled α-DC-ZZ-BNC complex and subjected to flow cytometric analysis. Fractions of DCs were pre-defined by the forward scatter/side scatter dot plots derived from CD11c+ cells. Distributions of Cy5-derived fluorescence in ZZ-BNC-incubated DCs and untreated DCs are indicated as closed and open histograms, respectively. Antibodies against DCs are shown in the upper left of each panel. The percentages (%) of ZZ-BNC+ cells in DCs are indicated as numbers. ( B ) Mean fluorescent intensities of the Cy5-labeled α-DC-ZZ-BNC complexes in DCs. Notes: Mean fluorescent intensity derived from DCs incubated with Cy5-labeled BNCs was defined as 100. Measurements were performed in triplicate. Error bars represent the SD. Abbreviations: DC, dendritic cell; ZZ-BNC, BNC displaying ZZ domains; DC, dendritic cell; SD, standard deviation.
Article Snippet: DCs (2.5–5.0 × 10 5 cells) were mixed with each
Techniques: Ex Vivo, Labeling, Isolation, Incubation, Derivative Assay, Fluorescence, Standard Deviation
Journal: International Journal of Nanomedicine
Article Title: Engineered hepatitis B virus surface antigen L protein particles for in vivo active targeting of splenic dendritic cells
doi: 10.2147/IJN.S32813
Figure Lengend Snippet: Flow cytometric analysis of in vivo attachment of α-DC-ZZ-BNC complexes to splenic DCs. ( A ) Distributions of Cy5-derived fluorescence in splenic DCs isolated from Cy5-labeled α-DC-ZZ-BNC complex-injected mice and untreated mice are indicated by closed and open histograms, respectively. ( B ) Distributions of Cy5-derived fluorescence in splenic CD11c − cells isolated from Cy5-labeled α-DC-ZZ-BNC complex-injected mice. Notes: The percentages (%) of ZZ-BNC + cells in DCs and CD11c − cells are indicated as numbers. Abbreviations: DC, dendritic cell; ZZ-BNC, BNC displaying ZZ domains.
Article Snippet: DCs (2.5–5.0 × 10 5 cells) were mixed with each
Techniques: In Vivo, Derivative Assay, Fluorescence, Isolation, Labeling, Injection
Journal: International Journal of Nanomedicine
Article Title: Engineered hepatitis B virus surface antigen L protein particles for in vivo active targeting of splenic dendritic cells
doi: 10.2147/IJN.S32813
Figure Lengend Snippet: Incorporation of Cy5-labeled α-CD11c (clone N418)-ZZ-BNC complexes by splenic DCs isolated from Cy5-labeled α-DC-ZZ-BNC complex-injected mice. Notes: Z-stack projections of splenic DCs were generated from deconvolved slices using the maximum intensity criteria. Fluorescence derived from ZZ-BNCs and CD11c molecules is indicated in red and green, respectively. Scale bars, 5 μm. Abbreviations: DC, dendritic cell; ZZ-BNC, BNC displaying ZZ domains; DIC, differential interference contrast.
Article Snippet: DCs (2.5–5.0 × 10 5 cells) were mixed with each
Techniques: Labeling, Isolation, Injection, Generated, Fluorescence, Derivative Assay